Ecotourism plays an important role in optimizing industrial structure, encouraging regional sustainable development, and promoting ecological conservation. Taking Huangshan Mountain scenic area as a case for studying mountain ecotourism: Firstly we introduce the abundant ecotourism resources in Huangshan Mountain scenic area, including wild animals and plants, famous and ancient trees; Secondly, we comb the relevant measures that are taken to promote ecotourism development regarding ecotourists, resources, enterprises, and environment in Huangshan Mountain scenic area, including the determination of reasonable carrying capacity of scenic spots, the closure of the elite scenic spots, the regularly protection of ancient and famous trees, the effective prevention and control of pine wood nematode disaster, the strict prevention of forest fires, the reform of eco-tourism enterprises and the scientific management of 'three waste' and so on; Thirdly, we discuss the economic, social, and ecological benefits of ecotourism development in Huangshan Mountain scenic area, its benefits have the spillover of time and space, which is playing an important role in promoting the sustainable development of domestic and foreign tourism destinations. Huangshan Mountain scenic area is an excellent example of ecotourism development in China, its unique ecotourism preservation and practice measures on regional development, legislation, and multi-stakeholder participation provide valuable experiences for the ecotourism development in the same type of scenic areas in China and abroad.